Basic culture information

John &/or Pam Bohmfalk bohmfalk at tcgcs.com
Tue Sep 19 18:17:12 EST 1995


I would like to use Chlamydomonas in a couple of undergraduate cell
biology labs, but am having difficulties growing it.  I purchased a
culture from Carolina, and am using their Chlamydomonas culture medium (50
mL in a 250 mL flask), and have the cultures at room temperature under
fluorescent lights in a plant growth chamber.  

Is this a decent medium?  Are my conditions OK?  To what density should
the cells grow?  How quickly?

Any help would be appreciated.




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