B.J. Rao wrote,
>We have been having very poor mating reactions between cc125(+)
>and cc1952(-) and resultant poor yield of tetrad yielding zygotes.
>Does anyone have any suggestions to improve this or any useful thoughts
>that might help us out? Is it a common problem associated with 1952 since
>it is a polymerphic ? Any input here will be highly appreciated.
>We have been following exactly the same procedure that is described in
>"Cilia & Flagella" volume of Methods in Cell Biology which I believe is
>a standard one.
I've never tried to do tetrads with this strain myself, although I have
verified that it does mate with CC-125. There are some suggestions for
trouble-shooting poor mating reactions on p. 173 of the Chlamy Sourcebook.
In particular, have you checked to see that you can get satisfactory mating
and tetrad viability from a control cross, e.g. CC-125 x CC-124?
chlamy at acpub.duke.edu