mating problems with cc1952

Elizabeth Harris chlamy at acpub.duke.edu
Thu Aug 29 12:58:54 EST 1996


B.J. Rao wrote,

>We have been having very poor mating reactions between cc125(+)
>and cc1952(-) and resultant poor yield of tetrad yielding zygotes.
>Does anyone have any suggestions to improve this or any useful thoughts
>that might help us out? Is it a common problem associated with 1952 since
>it is a polymerphic ? Any input here will be highly appreciated.
>We have been following exactly the same procedure that is described in
>"Cilia & Flagella" volume of Methods in Cell Biology which I believe is
>a standard one.

I've never tried to do tetrads with this strain myself, although I have
verified that it does mate with CC-125.  There are some suggestions for
trouble-shooting poor mating reactions on p. 173 of the Chlamy Sourcebook.
In particular, have you checked to see that you can get satisfactory mating
and tetrad viability from a control cross, e.g. CC-125 x CC-124?


Elizabeth Harris
chlamy at acpub.duke.edu






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