Dear Chlamy people!
I would greatly appreciate your advice concerning the following
problem. I am involved in electrophysiological studies of
photosensory transduction in Chlamy. We use a whole-cell suction
pipette technique, which means that the amplitude of the measured
electric signal is proportional to the cell size.
For our measurements, we convert cells of CW2 strain grown on agar
plates to gamets by overnight incubation in a low salt liquid
medium. The gamets appear to be very diverse in size and a fraction
of the bigger ones in particular preparation is hardly predictable.
Are there any ways to promote an increase in the cell size in
Chlamydomonas culture?
Looking forward for any suggestions,
best regards,
Elena Govorunova.
Elena.Govoru at vkl.uni-regensburg.de
