Protocol for nuclei prepn

bjrao at bjrao at
Wed Oct 2 09:29:02 EST 1996

Dear Chlamy folks:

Can someone direct me to a protocol that yields good quality
nuclei from Chlamy that contain cell-wall ? I assume that autolysin
treatment followed by NP-40 or anyother detergent lysis should yield
intact nuclei that can be pelleted. I would appreciate if someone can
share with me a lab-protocol or a published protocol that works for
nuclei isolation. Thanks:


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