Culture Media Recipes

Andy J A.M.JOHNSTON at dundee.ac.uk
Mon Feb 24 09:27:10 EST 1997


I wonder if anyone can help me.  I am having a few problems making up
bulk media for chlamydomonas reinhardtii.  I really need to be able to
make up the media in a block to avoid having to add anything after
autoclaving, apart from my innoculum.  I also want to avoid the use of
non-specific compounds such as "yeast extract" or "lab-lemco powder".
This is so I can calculate the osmolarity of the medium from the
molecular weights of the added compounds.  In brief the specifications
I require are as follows;

a.      "All in one" mix that will not precipitate on autoclaving.

b.      Use of only chemically defined ingredients (no yeast extract).

c.      "Complete" media for rapid and sustained growth.

Any ideas?

Karl






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