Sequencing Chlamydomonas

Joy Kovar jlk at unlinfo.unl.edu
Fri Oct 31 15:27:15 EST 1997


Hello,
        I am Joy Kovar working at the University of Nebraska-Lincoln.  Our
Sequencing Core Facility has been having a lot of trouble sequencing our
Chlamydomonas sequence due to the high GC content.  Has anyone had
similar problems?  If anyone has any ideas about how we might go about
trouble-shooting this problem, I'd be very grateful.

        You can contact me at the following e-mail address:
                jlk at unlinfo.unl.edu

        Thank you,
                Joy


------

Moderator's note:  This question came up a couple of years ago, and the
responses then were summarized by Emma Berta Gutierrez, for the
Chlamydomonas Genetics Center web page:

http://www.botany.duke.edu/DCMB/methods/sequence.htm


Have we made any progress in the past two years?  Since this is a question
of general interest to our readership, I encourage respondents to post
their answers to the whole list.


Elizabeth Harris
chlamy at acpub.duke.edu






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