Hello,
I am Joy Kovar working at the University of Nebraska-Lincoln. Our
Sequencing Core Facility has been having a lot of trouble sequencing our
Chlamydomonas sequence due to the high GC content. Has anyone had
similar problems? If anyone has any ideas about how we might go about
trouble-shooting this problem, I'd be very grateful.
You can contact me at the following e-mail address:
jlk at unlinfo.unl.edu
Thank you,
Joy
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Moderator's note: This question came up a couple of years ago, and the
responses then were summarized by Emma Berta Gutierrez, for the
Chlamydomonas Genetics Center web page:
http://www.botany.duke.edu/DCMB/methods/sequence.htm
Have we made any progress in the past two years? Since this is a question
of general interest to our readership, I encourage respondents to post
their answers to the whole list.
Elizabeth Harris
chlamy at acpub.duke.edu
