Myc fusion proteins

Rip J. Finst rfinst at emory.edu
Thu Mar 19 09:58:15 EST 1998


Hi,

We are considering inserting a myc epitope into a C. reinhardtii gene and
transforming this recombinant clone back into cells.  Ultimately, we'd
like to use antibodies against this epitope for immunolocalization.
Anybody have such experience with myc tagging C. reinhardtii proteins?
Are there endogenous proteins that are recognized as antigenic by anti-myc
antibodies (by Western analysis, immunofluorescence, or another method)?
How realistic is our approach?

Thanks,
Rip

----------------------------------
Rip Finst
Dept. of Cell Biology
Emory University School of Medicine
Atlanta, GA  30322
phone: (404) 727-8739
fax: (404) 727-6256






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