>Dear Netters,
>> Has anyone used fungicides, such as Nystatin or Amphotericin, to
>control
>fungal contaminants on TAP plates of Chlamy?
>Thanks.
>
There's a nystatin-resistant mutant, which suggests that this might not be
the fungicide of choice for wild-type contamination control. The reference
is Bard et al., Lipids 13, 533-539 (1978). We have the mutant, but I've
never tried to work with it. If somebody's interested in checking it out,
I'd be happy to send it.
There are some other suggestions for fungal cleanup, in response to a
bionet discussion a couple of years ago, on the web at:
http://www.botany.duke.edu/DCMB/methods/cleanup.htm
I usually find that it's not very difficult to clean up fungus
contamination, using a dissecting microscope and a glass needle. The
method is on the web page also, and in the Chlamy Sourcebook on pages
47-48.
Elizabeth Harris
chlamy at acpub.duke.edu
Elizabeth Harris
chlamy at acpub.duke.edu
