Postdoctoral Opportunity

Carl Johnson carl.h.johnson at vanderbilt.edu
Mon Nov 29 18:24:38 EST 1999

Postdoctoral Position in Protein Interactions
Vanderbilt University
Nashville, Tennessee, USA

A postdoctoral position is available April 1, 2000 to develop a new technique
for assaying protein-protein interactions in situ. This technique assays the
proximity of fusion proteins by Bioluminescence Resonance Energy Transfer
(BRET). Preliminary studies characterizing the BRET technique in bacteria are
described in: 

Xu, Y., D. Piston, and C.H. Johnson. 1999. A bioluminescence resonance energy
transfer (BRET) system: Application to interacting circadian clock proteins.
Proc. Natl. Acad. Sci. USA 96: 151-156.

The candidate will focus on extending the BRET technique to mammalian cells in
culture. Because our lab primarily studies the molecular and cellular basis of
circadian (daily) rhythmicity, part of the project will also utilize the
technique to examine the interaction of circadian clock proteins over the
daily cycle in situ. Salary will be consistent with the NIH scale. Support for
this position comes from an NIH grant that begins April 1, 2000 (pending final
approval of a grant proposal that received a high priority score) and ends
March 31, 2002, but the position will be continued if the grant is renewed
and/or the candidate can apply for their own postdoctoral fellowship after
beginning the project. Required: experience with molecular genetic techniques.
Desirable: experience with transfection of mammalian cells in culture, other
protein interaction assays, and/or microscopic techniques. Interested
applicants should send a current CV including the names of three references
to: Dr. Carl Johnson, Dept. of Biology, Box 1812-B, Vanderbilt University,
Nashville, TN  37235 USA.
email: carl.h.johnson at vanderbilt.edu
website: http://johnsonlab.biology.vanderbilt.edu.

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