I have some questions for any Chlamy transformation experts.
I had my undergraduate plant phys class do a chlamy tranformation
using the nit-1-305 strains and pMN24 plasmid, according to
Carol Reiss' plant phys book. It worked very well and I plan
on doing it again.
I would now like to put in a gene that I have been working with.
Can I clone it onto the pMN24 plasmid and use the nitrogen
selection? (I do think this particular gene could be a problem
for chlamy.)I would like any suggestions and ideas that anyone
University of Colorado
Colorado Springs, CO, USA
slblowe at mail.uccs.edu