Chlamydomonas light microscopy

Irene Baroli ibaroli at nature.berkeley.edu
Mon Jun 11 13:33:22 EST 2001


Dear Chlamy colleagues,

I would like to stain living Chlamy cells with a dye that detects reactive
oxygen species and need to know how to stop the cells from swimming without
killing them, to visualize the dye by light microscopy and take pictures.
Any hints will be greatly appreciated. Also, if you already have a protocol
for staining these cells with CM-H2DCFDA or any other reactive oxygen dye
that you'd be willing to share with me I will be most grateful!

Irene Baroli

__________________________________________________

Irene Baroli, Ph D
Dept. of Plant and Microbial Biology
University of California
111 Koshland Hall
Berkeley, CA 94720-3102 USA
Tel. 510-643-6604
Fax  510-642-4995
ibaroli at nature.berkeley.edu


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