Hello all,
I am attempting some experiments in which I need to do nuclear
transformation with the PDS-1000/He gene gun of chlamy. I have done a large
amount of shooting, but have had no good results. I've tried shooting on
cells imbedded in agar and agarose, cells actually grown up on the plates,
and cells on nylon membranes (the best candidate thus far), but have had no
success. I've also tried a variety of pressure disks and target locations,
macrocarrier locations, and microparticle preparation methods. I have tried
both the pJD67 (arg plasmid) and pSP124s (ble plasmid), but have had no
discernable transformants with either. I'm starting to get pretty
desperate, so any help would be greatly appreciated. Thanks.
Sincerely,
Andy Vaughan
fleaghost at hotmail.com
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