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David L Herrin DLHerrin at utxvms.cc.utexas.edu
Tue Aug 20 14:08:54 EST 2002


Dear Colleagues,

We are looking for a cell wall-deficient strain that gives a high rate of 
nuclear transformation with cosmids, but one that transforms well with 
plasmids will probably do. Also, the strain can't be spectinomycin 
resistant, which, strangely enough, CC3395 (arg7-8 cwd) apparently is. Our 
CC425 (arg 7-8 cw 15 mt+) strain is not growing all that well, and CC424 
(arg 7-8 cw15 mt-) transforms with moderate efficiency. Thanks for any 
offers or suggestions.

Sincerely,
David Herrin

Dr. David L. Herrin, Professor
Section of Molecular Cell and Developmental Biology
University of Texas at Austin
1 Texas Longhorns #A6700
Austin, Texas  78712 





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