autolysin

Lars-Gunnar Franzén Lars-Gunnar.Franzen at set.hh.se
Wed Mar 5 11:21:22 EST 2003


Dear Chlamy community,

I'm going to isolate gamete autolysin.

According to the Chlamydomonas Sourcebook (page 593), wild-type cells of 
both mating types should be pregrown in liquid phototrophic cultures, and 
then transferred to nitrogen-free medium.

Is the choice of growth medium important? Phototrophic or mixotrophic 
growth (i.e. without or with acetate)? High-salt minimal medium, HSA or TAP?
Is "nitrogen-free medium" HS, HSA or TAP with no ammonium chloride?

Regards,

Lars-Gunnar Franzén

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