freezing Chlamy?

Elizabeth Harris chlamy at duke.edu
Wed Nov 17 10:21:13 EST 2004


>Hi -
>I'm looking for information on freezing Chlamy cells.  How have 
>people been doing it recently, and with what kind of success?  Who 
>has been working on it?
>Thanks so much!!
>Kim, Marshall Lab
>
>---

The best method so far is to use methanol as a cryoprotectant and 
store the frozen cells in liquid nitrogen.  Using a -80 freezer 
doesn't work nearly as well.

Dick Sayre's freezing protocol is on line at

http://www.chlamy.org/methods/freezing.html

We've tested it here at Duke, and it works well.

Jerry Brand and Alexandra Crutchfield at the University of Texas 
algal collection (UTEX) have used a similar procedure with Chlamy and 
with some other unicellular algae, also with satisfactory results.


-- 
Elizabeth Harris

Chlamydomonas Center - new web site: http://www.chlamy.org/
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://iubio.bio.indiana.edu/bionet/mm/chlamy/attachments/20041117/8bf6caa8/attachment.html


More information about the Chlamy mailing list