>Hi -
>I'm looking for information on freezing Chlamy cells. How have
>people been doing it recently, and with what kind of success? Who
>has been working on it?
>Thanks so much!!
>Kim, Marshall Lab
>>---
The best method so far is to use methanol as a cryoprotectant and
store the frozen cells in liquid nitrogen. Using a -80 freezer
doesn't work nearly as well.
Dick Sayre's freezing protocol is on line at
http://www.chlamy.org/methods/freezing.html
We've tested it here at Duke, and it works well.
Jerry Brand and Alexandra Crutchfield at the University of Texas
algal collection (UTEX) have used a similar procedure with Chlamy and
with some other unicellular algae, also with satisfactory results.
--
Elizabeth Harris
Chlamydomonas Center - new web site: http://www.chlamy.org/
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