IUBio

pArg7.8 sequence

lkeller at itsa.ucsf.edu lkeller at itsa.ucsf.edu
Thu Nov 18 15:26:24 EST 2004


I am looking for anyone who has the sequence of the pArg7.8 plasmid that is 
frequenty used for insertional mutagenesis. The original reference
(Debuchy et al., 
1989) doesn't seem to tell me what the original plasmid is and I'm also
not sure how 
much of the Arg gene was put in. I need to know where in the plasmid NruI 
cuts. 
Any comments would be greatly appreciated. Thanks!!

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