Arthur Grossman has sent the following announcement. Please share it
with other people in your lab, since not everyone is subscribed to
this list/newsgroup.
THE NEW ARRAY IS COMING!
The version 2 microarray has just been printed and an initial test
has been performed. This array consists of about 10,000
oligonucleotides, each approximately 70 nucleotides long. Each of
the oligonucleotide should represent a unique gene. The set of
10,000 oligonucleotides was printed twice on the same slide (so each
slide has duplicates). The oligonucleotides were prepared by
Integrated DNA Technologies and their quality was checked by mass
spectroscopy. The set of 'unigenes sequences' used for
oligonucleotide generation was developed by Monica Jain and Stephan
Eberhard in my laboratory. The initial tests, performed by Stephan
Eberhard, are currently being analyzed; additional testing will be
performed over the next 2-3 weeks to insure that the quality is high
and that the .gal file provides the proper sequence locations. We
will also provide information describing potentially empty and weak
spots (there were very few of these in our initial examination).
While the .gal file with a gene identifier and the sequence of the
oligonucleotide used at each position on the array will be available,
it may take a little more time to link whatever annotation is
available to the individual spots (it will be a priority over the
next month or two). We will also put a protocol onto the web site
(by the end of the month) that we used for the hybridizations and
will try to address any technical questions that you might have
concerning the array. A manuscript describing how the unigene set
was chosen and oligonucleotide design is in preparation, and we will
make it available to people using the array as soon as possible.
We will start to take orders for the new array now, and the shipping
of the arrays will begin by the middle of March (it depends a little
on how the tests go). Please send orders to:
Arthur Grossman
Department of Plant Biology
Carnegie Institution of Washington
260 Panama Street
Stanford, CA 94305
Email: arthurg at stanford.edu
Telephone: 650 325-1521 X 212
If you have any questions contact me by Email or phone.
Cheers,
Arthur
--
Elizabeth H. Harris
Chlamydomonas Center - new web site: http://www.chlamy.org/
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