[Chlamydomonas] Transient expression with CMV promoter?

Peter Luykx pluykx at bio.miami.edu
Wed Jun 15 15:55:18 EST 2005


15 June 2005

A colleague of mine asks the following question.  You can forward any 
replies to him directly, or to me.  Thanks.  --- Peter Luykx, Univ. of 
Miami.*
*

    We want to transiently express a GFP-tagged Golgi enzyme in 
Chlamydomonas by particle bombardment (which we regularly do with higher 
plants). Can we use the 35S CMV promoter which we use for higher 
plants?  Does this work in Chlamy?  If not which is the appropriate 
promoter to use?

Prof. David G. Robinson
Heidelberger Institut für Pflanzenwissenschaften
Abteilung Zellbiologie
Im Neuenheimer Feld 230
69120 Heidelberg
Germany
Tel. +49 6221 546406
Fax +49 6221 546404

david.robinson at urz.uni-heidelberg.de 
<mailto:david.robinson at urz.uni-heidelberg.de>

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