[Chlamydomonas] growing chlamy on 24-well plates

collins at mpiz-koeln.mpg.de collins at mpiz-koeln.mpg.de
Fri Oct 21 05:17:53 EST 2005


Hi everyone,

I was wondering if anyone has any experience growing/maintaing chlamy
cultures in small volumes (say on a 12 or 24-well tissue culture plate)
for long periods of time. I'm planning a selection (natural selection)
expt and want to get many replicate populations. I'm a little worried that
the cultures will either splash and migrate from well to well if I put
them on a shaker, or will end up adapting to low gas levels if I don't
shake the cultures.

Sinead Collins



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