[Chlamydomonas] Strain contamination

Bernd, Karen kabernd at davidson.edu
Thu Feb 23 10:13:18 EST 2006


I work on flagella organization and swimming force generation and my
department uses chlamy mating and flagella regeneration in our intro course.
We have been having a reoccurring problem with contamination and I would be
very appreciative of any and all help.

We have a microbe that only grows in co-culture with Chlamy. It is resistant
to amp and kan and lives in Lugols but is stained black by it. ANY help in
identifying what the microbe is and how we can kill it (without killing the
chlamy) would be greatly appreciated.

Chlamy that are contaminated with it become deflagellated (about 1 in 100
have flagella) This makes it very hard for the intro students to look at
flagella regeneration and for my research students (also all undergrads) to
study flagella force generation when you have to search for the 1 in 100
cells that still have flagella.

I am in North Carolina where fungal contamination is the bane of an
undergrads existence. The contamination seems to reappear in August and I
use serial dilution and selecting for singles to tease cells away from
contaminants but would really like a quicker way. We use flow hood, have
EtOH washed surfaces and scrubbed down incubators with four different
detergents (including one nasty on from the animal care facility),
re-autoclaved, UV treated and still it comes back.

I am new to the list-- if this has been discussed before please point me to
the correct thread.

Thank you.
******
Karen K. Bernd, Ph.D.
Associate Professor of Biology
Davidson College
http://www.bio.davidson.edu/bernd

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