[Chlamydomonas] protein expression

Faezeh Koohestani via chlamy%40net.bio.net (by fkoohestani from gmail.com)
Mon Jul 23 10:17:00 EST 2007


Thanks for your response.
I think for functional analysis I need to express it in Xenopus oocytes or
mutant yeast.

Faezeh

On 7/22/07, Lutz Wobbe <lutz.wobbe from uni-bielefeld.de> wrote:
>
> Hi Faezeh,
>
> in which cell type would you like to express the transporter? In Chlamy?
> In a different organism?
>
> Best regards
>
> Lutz
>
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> > Message: 1
> > Date: Fri, 20 Jul 2007 16:11:04 -0500
> > From: "Faezeh Koohestani" <fkoohes2 from uiuc.edu>
> > Subject: [Chlamydomonas] protein expression
> > To: chlamy from magpie.bio.indiana.edu
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> > Hello,
> >
> > Does anyone have any suggestions for the best expression system
> > for a
> > Chlamy? I am going to clone and express a Chlamy transporter and am
> > concerned about post-transcriptional modifications as well as
> > cost. I would
> > appreciate any suggestions/advice.
> >
> > Thanks
> >
> >
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