Hi,
I am just beginning to work with Chlamy, and have had trouble getting
consistent cell counts. My current protocol consists of removing a sample
from my culture, spiking it with 10% Lugol's solution, and then counting
with a hemacytometer. When performing a growth curve, my counts are
inconsistent, especially at higher cell densities. This contrasts with OD
measurements made at 750 nm, which are beautiful. Any suggestions would be
greatly appreciated!
Jon
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