[Chlamydomonas] Need help to find a working mithramycin staining protocol for Chlamydomonas

s.kiaulehn from web.de via chlamy%40net.bio.net (by s.kiaulehn from web.de)
Mon Apr 28 10:33:21 EST 2008


Dear Chlamydomonas community,

I am PhD student working on Chlamydomonas reinhardtii and tried to
stain cw15 (mt-) cells with mithramycin, a dye for the nucleus I found
in the Chlamydomonas Source Book. I referred Bridget & Whatley (1975)
to strain the cells by adding 20 µg/ml mithramycin and 5 mM MgCl2
(final concentration). I incubated  the cells 20 min at room
temperature and in the absents of light before I watched them through
the microscope (Zeiss Axiophot, 450-490 nm excitation, 510 nm beam
splitter, 520 nm barrier filter).
Unfortunately I was not successful staining the cells. I only could
see strong red auto-fluorescent of chloroplasts but no yellow/green
fluorescent nucleus.

Is there anybody out there knowing a working mithramycin staining
protocol for Chlamydomonas who is willing inaugurate me into the
secret of staining cells with mithramycin?

Many thanks in advance!

Kind regards
Stefanie



More information about the Chlamy mailing list