From nicastro from brandeis.edu Fri Feb 8 00:33:33 2008 From: nicastro from brandeis.edu (Daniela Nicastro) Date: Fri Feb 8 08:22:06 2008 Subject: [Chlamydomonas] Postdoc & EM Technician Positions Message-ID: <000f01c86a14$25b46a50$6a214081@manta> Dear All, four positions are available immediately at Brandeis University: 1. A postdoctoral position is available in the laboratory of Dr. Nicastro to study cilia and flagella in the model organism Chlamydomonas or Tetrahymena, using molecular genetic approaches including RNAi technology, biochemistry and high-resolution imaging. Further details about this opportunity are available at: 2. A postdoctoral position is available in the laboratory of Dr. Nicastro to study the 3D structure and function of molecular motors using cryo-electron tomography (Nicastro et al. 2006 Science 313:944). Further details about this opportunity are available at: 3. We also have openings for two candidates (one Postdoc and one EM Technician) with experience in high-pressure freezing, freeze-substitution and ultramicrotomy. The successful candidates will join the newly implemented cellular visualization facility for correlative light and electron microscopy to work closely with cell and neurobiologists. Further details about this opportunity are available at: Interested candidates should contact nicastro@brandeis.edu. Best wishes, Daniela ******************************************** Dr. DANIELA NICASTRO MS 029, Rosenstiel Center Brandeis University 415 South Street Waltham, MA 02454-9110 Phone: (781) 736-2408 Fax.: (781) 736-2419 email: nicastro@brandeis.edu http://www.bio.brandeis.edu/nicastrolab/ http://www.futurehealth.ucsf.edu/scholars/07spages/nicastro.html ******************************************** From lb273 from cornell.edu Mon Feb 11 10:30:06 2008 From: lb273 from cornell.edu (Lutz Becks) Date: Mon Feb 11 10:57:22 2008 Subject: [Chlamydomonas] CellTracker Message-ID: <1677.132.236.111.16.1202743806.squirrel@webmail.cornell.edu> Hello, does anyone have an idea if it is possible label living Chlamy cells e.g. with CellTracker from Invitrogen. Thanks for your help. From xyraxx from hotmail.com Sun Feb 24 17:11:41 2008 From: xyraxx from hotmail.com (Ray Jee) Date: Mon Feb 25 21:48:08 2008 Subject: [Chlamydomonas] new to chlamydomonas and have a questions! Message-ID: Hello, My name is Raymond Jee I am a sophomore at Rio Rancho High School in Rio Ra= ncho, New Mexico. I am currently working on a science expo project; it invo= lves the hydrogen production of chlamydomonas with and without flagella. I = have a couple questions and was wondering if anyone could answer them? I cu= rrently have two strains of chlamydomonas one with flagella that I bought f= rom WardSci and another without flagella given to me courtesy of Peter Lefe= bvre from UMN. It turns out when transferring flagella less chlamydomonas f= rom an agar to a liquid they seem to culture slower. Is there something I= =92m not doing correctly? Do they need to be under continuous stirring or = bubbling because they have no flagella? Does anybody have an inexpensive me= thod to measure the hydrogen after it is produced? If you have any suggestions or ideas please e-mail me at xyraxx@hotmail.com =20 Thank you for your time=20 =20 Raymond Jee =20 _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/=