From yuan.li from adelaide.edu.au Tue Mar 4 23:19:33 2008 From: yuan.li from adelaide.edu.au (Yuan Li) Date: Wed Mar 5 12:12:19 2008 Subject: [Chlamydomonas] Does anyone know how efficent is it to use chloroform vapours to kill unmated cells? Message-ID: <6E1D2123B30349E29359D8401461198B@ad.adelaide.edu.au> Hi, Does anyone know how efficent is it to use chloroform vapours to kill unmated cells or how to improve the efficience? I exposed the zygote plate to chloroform for 3 minutes and it seems there still are unmated cells. Cheers Yuan From Jay.R.Thompson-1 from USA.dupont.com Fri Mar 7 15:21:14 2008 From: Jay.R.Thompson-1 from USA.dupont.com (Jay R Thompson) Date: Sat Mar 8 17:37:18 2008 Subject: [Chlamydomonas] SiC whiskers search Message-ID: Hello, My inquiry is in reference to transformation of walled Chlamydomonas cells mediated by silicon carbide whiskers and how to acquire the SiC whiskers for continuing work at DuPont. It would be helpful to have the name of a couple suppliers if SiC whiskers are still be used for this applications. thanks, Jay Thompson (302) 999-4617 This communication is for use by the intended recipient and contains information that may be Privileged, confidential or copyrighted under applicable law. If you are not the intended recipient, you are hereby formally notified that any use, copying or distribution of this e-mail, in whole or in part, is strictly prohibited. Please notify the sender by return e-mail and delete this e-mail from your system. Unless explicitly and conspicuously designated as "E-Contract Intended", this e-mail does not constitute a contract offer, a contract amendment, or an acceptance of a contract offer. This e-mail does not constitute a consent to the use of sender's contact information for direct marketing purposes or for transfers of data to third parties. Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean http://www.DuPont.com/corp/email_disclaimer.html From yuan.li from adelaide.edu.au Wed Mar 12 20:21:41 2008 From: yuan.li from adelaide.edu.au (Yuan Li) Date: Thu Mar 13 18:40:39 2008 Subject: [Chlamydomonas] About the vector Patp-int-cg12 Message-ID: Hi, Could someone please tell me if the Patp-int-cg12 vector was sequenced or not, and how can I find the sequence if it was? Thanks a lot. Yuan From raj from Ag.arizona.edu Sat Mar 15 14:40:55 2008 From: raj from Ag.arizona.edu (Rich Jorgensen) Date: Sat Mar 15 14:43:48 2008 Subject: [Chlamydomonas] The iPlant Collaborative: what potential does it have for advancing plant research? Message-ID: <89366D6D-F193-43EA-8B2C-7AF1F013A600@Ag.arizona.edu> Dear Chlamy researchers, I'm writing to encourage creative thinkers in your community invest a =20= little time in understanding the iPlant Collaborative = (www.iplantcollaborative.org=20 ) and thinking about what it might be able to do for the plant =20 sciences. To jumpstart your thinking process, you might want to =20 consider participating in the iPlant Collaborative's April kickoff =20 conference at Cold Spring Harbor Lab, either in person or via our =20 free, live webcast which will allow for direct participation (details =20= at our web portal). Participation in the conference is NOT necessary =20 for participation in the Collaborative, but may be helpful in =20 understanding how best to participate. International participation is =20= both welcome and encouraged. The first principle of the iPlant Collaborative =96 our "prime =20 directive", one might say =96 is that it must be "by, for and of the =20 community". A second major principle is that the iPC's =20 cyberinfrastructure designs must be driven by specific, compelling, =20 and tractable Grand Challenges in the plant sciences. A third major =20 principle is that the Collaborative must serve the entire breadth of =20 the plant sciences, including ecology, evolution and organismic =20 biology as much as the molecular, cellular and developmental =20 disciplines, and via Grand Challenges integrated across the 'divide', =20= from the molecular to the organismic to ecosystems. In order to ensure =20= Collaborative resources are dedicated to the most compelling Grand =20 Challenges in the Plant Sciences, the best and the brightest in plant =20= biology will need to invest time and provide leadership to ensure the =20= field assembles and submits the best possible GC proposals to iPlant's =20= external Board of Directors. Importantly, the project is NOT based on the idea of "if we build it, =20= they will come." Rather, the community must FIRST come together and =20 decide WHAT we should build, or no cyberinfrastructure will actually =20 be built. So, the first challenge we face is to engage the community =20 and convince those of you who think deeply about the important =20 questions in plant biology, as well as comprehend the real, down-and-=20 dirty details of data quality, availability and analysis, to identify =20= the most compelling and tractable Grand Challenges that require =20 computational approaches and cyberinfrastructure development. (see =20 iPlant's community wiki to contribute your discussion of what these =20 GC's ought to be.) Self-forming Grand Challenge Teams are the most direct way to =20 participate in the iPlant Collaborative. Any group can start a Grand =20 Challenge Team, or propose a Grand Challenge Workshop at which to =20 develop one. GC Teams are central to the iPlant Collaborative because =20= the community through its Board of Directors will choose which Grand =20 Challenges should be prioritized for cyberinfrastructure design and =20 development. Once GC Teams are chosen (our target is 2-4 GCT's before =20= late 2008/early 2009), the iPC's Integrated Solutions Team, led by =20 Lincoln Stein (CSHL) and Sudha Ram (UA), will work with each GCT to =20 design a 'Discovery Environment' to address a particular grand =20 challenge. Successful development of these prototype =20 cyberinfrastructures (Discovery Environments) will require close =20 interaction between IS Team and GC Team members. (See the Grand =20 Challenge Process tab at our web portal for more details.) We also =20 look forward to partnering with other CI efforts around the world. Self-forming Grand Challenge Teams do not need to wait for the =20 conference in April to get started. The conference is an opportunity =20 for plant and computing researchers to get together and so attendance =20= is one way to foster or participate in formation of GC Teams. It is =20 not obligatory for participation in the project (though we do hope to =20= have broad representation of the full range of plant biologists and =20 computing researchers so that discussions will be high quality and =20 balanced). The conference is NOT a bioinformatics meeting - it is a biology =20 conference aimed at defining which are the most compelling and =20 tractable grand challenges in the plant sciences that might benefit =20 from cyberinfrastructure development. The conference will be webcast =20 live, allowing for direct participation in discussions over the web =20 (and will be archived for later viewing). You can participate on your =20= laptop. Another suggestion I would offer would be for interested =20 organizations to arrange a webcast location (requiring only a =20 computer, web access and a projector) where local researchers could =20 come together to participate in and discuss the conference - we will =20 have facilitators to ensure all persons can participate in discussion. =20= Some institutions are also holding pre-meetings to discuss the =20 project: what it might mean for the campus and how to participate most =20= effectively in the Grand Challenge identification process which will =20 define the direction of the project. I hope the community will =20 consider participating substantively so it will not be left out of the =20= conversation, and so it will be positioned to participate prominently =20= in the Collaborative as it develops. (Program and pre-registration =20 links for both in-person and virtual attendance are at the project's =20 web portal.) To ensure community buy-in and ownership of the Collaborative, an =20 independent Board of Directors has been selected which will set =20 priorities for the allocation of Collaborative resources to particular =20= grand challenges, through a process involving self-forming grand =20 challenge teams that will arise from the community and make proposals =20= to the Board. The PI's will be available to facilitate the efforts of =20= GC teams, but we are agnostic about which grand challenges should be =20 prioritized. To ensure substantial independence, the Board of =20 Directors was appointed through a bootstrapping process, via a =20 Nominating Committee, not by the PI's. One third of the Board will =20 refresh annually. The composition of both the Board of Directors and the Nominating =20 Committee can be found at the project's web portal, = www.iplantcollaborative.org=20 . To date, the Board includes biologists Rob Last (chair), Sabeeha =20 Merchant, Jim Birchler, Toby Kellogg, Jose Arguello, Susan Singer, =20 Russ Monson, David Rand, Jean-Philippe Vielle, and Mark Westoby. An =20 equal number of Board members represents the computing research =20 community, from bioinformatics to computational biology to computer =20 science, information science, and computing infrastructure (Eric =20 Mjolsness, Steve Mayo, Fran Berman, Gwen Jacobs, Laurie Kirsch, Mohan =20= Tanniru), in order to be able to determine which proposals are really =20= tractable and to advise and guide Collaborative management. Thus, the =20= Board will possess diverse, balanced expertise with which to evaluate =20= any Grand Challenge proposal submitted by the community. The iPlant Collaborative is funded by NSF's Plant Sciences =20 Cyberinfrastructure Collaborative program in the Emerging Frontiers =20 division of BIO, as a $50M grant over 5 years to develop a =20 cyberinfrastructure for the plant sciences, from molecules, genes, and =20= cells to organisms, ecosystems and evolution. As plant biologists, we =20= are quite fortunate that our community has been given this unique =20 opportunity to lead biology cyberinfrastructure development in the =20 service of trying to solve biology=92s major, unanswered questions. The =20= reason the plant biology community has been entrusted with this =20 opportunity and responsibility is, I believe, because we have shown =20 exceptional openness, creativity and leadership across disciplines and =20= experimental organisms over many years. What better community than =20 plant scientists could NSF have chosen for this program? Also, had it =20= not been the plant sciences, these funds would presumably have gone =20 instead to areas of biology other than plant biology. So, this is an =20 extraordinary opportunity for the whole community, and one that we can =20= all feel proud to have earned. Feel free to pass this letter along to your colleagues. I look forward =20= to seeing many of you at CSHL, either online or in person, for what I =20= believe promises to be a pivotal event for plant biology. We are able =20= to waive onsite costs to increase diversity in the conference, so =20 please don't hesitate to ask if you feel you are in that category =20 (flexibly defined). More information can be found at www.iplantcollaborative.org, =20 including the NSF solicitation, our proposal, site visit questions and =20= answers, a ppt presentation, and other documents, as well as 1-2 page =20= backgrounders on different aspects of the project. I am available any =20= time to discuss the project. Rich Jorgensen Director, The iPlant Collaborative www.iplantcollaborative.org