From jflin from brandeis.edu Thu Dec 17 15:27:20 2009 From: jflin from brandeis.edu (Jianfeng Lin) Date: Thu Dec 17 15:40:49 2009 Subject: [Chlamydomonas] Does "LED Puck Light" works for Chlamydomonas? Message-ID: <661871931.6806551261081640447.JavaMail.root@zimbra-store-3.unet.brandeis.edu> Hi Colleague, I have a question about the light which will be used to culture the Chlamydomonas. Does "LED Puck Light" works for Chlamydomonas? This light produce little heat and seems to fit my requirement well. I need to culture Chlamydomonas in a sealed hood which can make sure the light/dark circle. So the heat from the light become a problem. Does anybody use this light for Chlamydomonas or have some suggestion? -- Nicastro Lab Rosenstiel Center, MS029 Brandeis University 415 South Street Waltham, MA 02454-9110 Phone: (++1) 781 736 2439 From RKurtz from commack.k12.ny.us Sun Dec 20 08:42:41 2009 From: RKurtz from commack.k12.ny.us (Richard Kurtz) Date: Sun Dec 20 09:08:36 2009 Subject: [Chlamydomonas] from a high school teacher Message-ID: <43E486C1898A4A4AB8F458C7089A86261D25994592@Commail2.commack.k12.ny.us> To whom it may concern, Some high school students of mine have been trying to develop a project involving aspects of biofuels and algae. The experimental idea was to find an Algae that we could grow in our classroom, algae that would lend itself to relatively easy culturing, that we would be able to keep in our lab at school. We want to have as an independent variable changing the carbon (sugar) source concentration of the alga. We want to measure the oil content (dependent variable) in the algae given different carbon sources. Measuring the oil content may be hard, we thought we could use a stain called nile red a stain for oils I think. Then use a microscope to take pictures of cells and identify the oil and use a computer program called image j to calculate the area of the oil. Of course I am not sure if this is possible of if there is another way to estimate oil concentration in the cells. I am writing to this forum to see if anyone could give us some feedback on the plan and if anyone has suggestions for us, in terms of culturing methods, manipulating a carbon source and measuring lipids in algae. Any suggestions or ideas would be greatly appreciated. Thank you, Richard Kurtz Commack High School Long Island, NY