From MitchelD from upstate.edu Tue Oct 6 07:34:41 2009 From: MitchelD from upstate.edu (David Mitchell) Date: Tue Oct 6 13:55:08 2009 Subject: [Chlamydomonas] Chlamy Meeting June 6-June 10, 2010: SAVE THE DATE Message-ID: <4ACB0121020000440004C4DE@gwmta2.upstate.edu> Dear Colleague: We hope you will be interested in attending the 2010 Chlamy meeting, which will be held in the greater Boston area next June. Watch for more announcements as we develop the final CHLAMY2010 program. Please add the dates to your calendar! 14th International Conference on the Cell and Molecular Biology of Chlamydomonas Wheaton College, Norton, Massachusetts Sunday June 6 - Thursday June 10, 2010 Keynote Speaker: Ritsu Kamiya Topics to be included: Gene Expression and microRNAs Chloroplasts and Mitochondria Flagellar Structure, Motility and Signaling Biofuels and Metabolism The Intraflagellar Transport system and Flagellar Assembly Photosynthesis Light Perception and Photoresponses Circadian Rhythms and the Cell Cycle Adaptation and Stress Responses Basal Bodies and Centrioles Development and the Sexual Cycle Evolution Organizers: David Mitchell (Upstate Medical University, Syracuse, NY, USA) Stephen Miller (University of Maryland Baltimore County, Baltimore, MD, USA) Contact: David R. Mitchell, Ph.D. Department of Cell and Developmental Biology, SUNY Upstate Medical University 750 E. Adams St. Syracuse, NY 13214, USA T-315-464-8575 FAX-315-464-8535 Email:mitcheld@upstate.edu From Olivier.Vallon from ibpc.fr Tue Oct 13 11:05:47 2009 From: Olivier.Vallon from ibpc.fr (Olivier Vallon) Date: Tue Oct 13 14:08:43 2009 Subject: [Chlamydomonas] growing and mating Chlamy in 96-well plates Message-ID: <4AD4A55B.4040602@ibpc.fr> Dear Chlamy community I'm trying to grow and mate Chlamy in 96-well plates. I'd be curious to hear from other people who have experience in this area. My personnal experience with flat bottom plates is that the cells tend to form a ring at the bottom, and I don't think it's good if you want to reach decent cell densities or see your cells survive for a decent period of time. Has anyone compared round-bottom or V-shaped plates ? Any feelings about plastic ? Polystyrene, vs. polyethylene, vs. special plastics (e.g. Nunc Microwell low-binding) ? I'd be tempted to go for a low-binding plastic, because Chlamy likes to swim around, but do the cheaper plates work ? Is agitation possible at all in these small wells ? How do you control evaporation, especially of the outermost wells ? What about large-volume wells ? I tried 1.3 ml and 2 ml Deepwell plates, but the cell density remained low so I never gained much in terms of yield. The surface/volume ratio becomes terrible, and the opaque plastic reduces light access. The cells tends to fall to the bottom. Has anyone had more success ? And mating ? There are quite a few labs out there who do matings in 96-well format, but I'm not sure what type of plate they use, and how they place the cells in gametogenesis conditions. Does diluting from TAP into water or N-free medium works ? Has anyone succeeded in storing the zygotes in the wells ? I myself have used cat litter, but its handling becomes quite messy in a microtiter plate format. Do glass beads work ? Thanks for your answers, and other thoughts on this not-so-trivial subject Olivier Vallon -- Olivier VALLON CNRS, UMR 7141 CNRS/Universit? Pierre et Marie Curie Institut de Biologie Physico-Chimique 13 rue Pierre et Marie Curie 75005 Paris FRANCE ovallon@ibpc.fr tel: (33) (0)1 5841 5058 fax: (33) (0)1 5841 5022 http://www.ibpc.fr/UMR7141/ From sarbiot from gmail.com Fri Oct 23 11:43:11 2009 From: sarbiot from gmail.com (SARAH) Date: Fri Oct 23 14:38:08 2009 Subject: [Chlamydomonas] BAC Message-ID: Do you know how to search BAC of Chlamy genome on the web please?