From frej.tulin from gmail.com Tue Sep 1 17:02:12 2009 From: frej.tulin from gmail.com (Frej Tulin) Date: Tue Sep 1 20:04:23 2009 Subject: [Chlamydomonas] replica plating Message-ID: Dear all Does anyone have a good method for replica plating Chlamydomonas plates? I am currently using the standard budding yeast method with a velveteen. I find that the colonies tend to 'smear out', which makes it difficult to distinguish individual colonies, especially when the plate has a lot of colonies. Is there a filter paper or another material that gives better result? Many thanks! /frej From chlamy from duke.edu Wed Sep 2 06:30:27 2009 From: chlamy from duke.edu (Elizabeth Harris) Date: Wed Sep 2 06:56:44 2009 Subject: [Chlamydomonas] replica plating In-Reply-To: References: Message-ID: >Does anyone have a good method for replica plating Chlamydomonas plates? Whatman #1 filter paper works much better for Chlamy than velveteen. There's a detailed description of how to go about it, including photographs, in the first edition (1989) of The Chlamydomonas Sourcebook, pages 42-47. A shorter version of the same text appears in the second edition, volume 1, pages 255-256. -- Elizabeth H. Harris Chlamydomonas Center http://www.chlamy.org/ From kabernd from davidson.edu Wed Sep 2 12:25:06 2009 From: kabernd from davidson.edu (Bernd, Karen) Date: Wed Sep 2 15:28:21 2009 Subject: [Chlamydomonas] Re: Chlamy Digest, Vol 46, Issue 1 In-Reply-To: <200909021705.n82H5Cp06883@net.bio.net> Message-ID: Frej- It also depends on the purpose for replica plating. I have used 96 well plates for stock plates for years. I use a multichannel pipettor to fill the wells with whatever media you choose and a 96 pin applicator or an 8channel pipettor to transfer samples from an 'old' plate to a fresh one. If you just need to transfer a small amount (dot blot/ lysing for enzymatic assay etc) this may also work, but Whatman paper is probably better. I know for certain that it cuts down stock maintenance time ****** Karen K. Bernd, Ph.D. Co-director Merck/AAAS Undergraduate Science Research Program Associate Professor of Biology at Davidson College http://www.bio.davidson.edu/bernd No trees were harmed in the typing of this email, but thousands of electrons were terribly inconvenienced. Please don't print this e-mail unless you really need to. ________________________________ From: Reply-To: Date: Wed, 2 Sep 2009 13:05:12 -0400 To: Subject: Chlamy Digest, Vol 46, Issue 1 Send Chlamy mailing list submissions to chlamy@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/chlamy or, via email, send a message with subject or body 'help' to chlamy-request@net.bio.net You can reach the person managing the list at chlamy-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Chlamy digest..." Today's Topics: 1. replica plating (Frej Tulin) 2. Re: replica plating (Elizabeth Harris) ---------------------------------------------------------------------- Message: 1 Date: Tue, 1 Sep 2009 18:02:12 -0400 From: Frej Tulin Subject: [Chlamydomonas] replica plating To: chlamy@magpie.bio.indiana.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear all Does anyone have a good method for replica plating Chlamydomonas plates? I am currently using the standard budding yeast method with a velveteen. I find that the colonies tend to 'smear out', which makes it difficult to distinguish individual colonies, especially when the plate has a lot of colonies. Is there a filter paper or another material that gives better result? Many thanks! /frej ------------------------------ Message: 2 Date: Wed, 2 Sep 2009 07:30:27 -0400 From: Elizabeth Harris Subject: Re: [Chlamydomonas] replica plating To: chlamy@magpie.bio.indiana.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" >Does anyone have a good method for replica plating Chlamydomonas plates? Whatman #1 filter paper works much better for Chlamy than velveteen. There's a detailed description of how to go about it, including photographs, in the first edition (1989) of The Chlamydomonas Sourcebook, pages 42-47. A shorter version of the same text appears in the second edition, volume 1, pages 255-256. -- Elizabeth H. Harris Chlamydomonas Center http://www.chlamy.org/ ------------------------------ _______________________________________________ Chlamy mailing list Chlamy@net.bio.net http://www.bio.net/biomail/listinfo/chlamy End of Chlamy Digest, Vol 46, Issue 1 ************************************* From skuehn from mail.rockefeller.edu Wed Sep 23 18:43:35 2009 From: skuehn from mail.rockefeller.edu (Seppe Kuehn) Date: Wed Sep 23 19:54:29 2009 Subject: [Chlamydomonas] gametes Message-ID: <33DF6426-F63E-45BE-ABD1-46A12F667EBF@rockefeller.edu> I am wondering if anyone knows a simple way to detect the presence of gametes in a population of chlamydomonas reinhardtii _without_ running a mating reaction. are there any cytometric methods known for this? also, i am wondering if anyone has any experience studying populations of gametes that are not permitted to mate. in the literature it is clear that gametes can survive nutrient deficiency for long periods. has anyone studied this process systematically? thank you very much, Seppe Kuehn The Rockefeller University