[Chlamydomonas] Re: Chlamy Digest, Vol 46, Issue 1

Bernd, Karen via chlamy%40net.bio.net (by kabernd from davidson.edu)
Wed Sep 2 12:25:06 EST 2009

It also depends on the purpose for replica plating. I have used 96 well plates for stock plates for years. I use a multichannel pipettor to fill the wells with whatever media you choose and a 96 pin applicator or an 8channel pipettor to transfer samples from an 'old' plate to a fresh one.

If you just need to transfer a small amount (dot blot/ lysing for enzymatic assay etc) this may also work, but Whatman paper is probably better. I know for certain that it cuts down stock maintenance time
     Karen K. Bernd, Ph.D.
Co-director Merck/AAAS Undergraduate Science Research Program
Associate Professor of Biology at Davidson College

No trees were harmed in the typing of this email,
but thousands of electrons were terribly inconvenienced.
Please don't print this e-mail unless you really need to.

From: <chlamy-request from oat.bio.indiana.edu>
Reply-To: <chlamy from oat.bio.indiana.edu>
Date: Wed, 2 Sep 2009 13:05:12 -0400
To: <chlamy from magpie.bio.indiana.edu>
Subject: Chlamy Digest, Vol 46, Issue 1

Send Chlamy mailing list submissions to
        chlamy from net.bio.net

To subscribe or unsubscribe via the World Wide Web, visit
or, via email, send a message with subject or body 'help' to
        chlamy-request from net.bio.net

You can reach the person managing the list at
        chlamy-owner from net.bio.net

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Chlamy digest..."

Today's Topics:

   1. replica plating (Frej Tulin)
   2. Re: replica plating (Elizabeth Harris)


Message: 1
Date: Tue, 1 Sep 2009 18:02:12 -0400
From: Frej Tulin <frej.tulin from gmail.com>
Subject: [Chlamydomonas] replica plating
To: chlamy from magpie.bio.indiana.edu
        <c8dd4ce40909011502u867b1a6hf6e6e9146b20077a from mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Dear all
Does anyone have a good method for replica plating Chlamydomonas plates? I
am currently using the standard budding yeast method with a velveteen. I
find that the colonies tend to 'smear out', which makes it difficult to
distinguish individual colonies, especially when the plate has a lot of

Is there a filter paper or another material that gives better result?
Many thanks!


Message: 2
Date: Wed, 2 Sep 2009 07:30:27 -0400
From: Elizabeth Harris <chlamy from duke.edu>
Subject: Re: [Chlamydomonas] replica plating
To: chlamy from magpie.bio.indiana.edu
Message-ID: <p06200700c6c40722d5ed@[]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

>Does anyone have a good method for replica plating Chlamydomonas plates?

Whatman #1 filter paper works much better for Chlamy than velveteen.
There's a detailed description of how to go about it, including
photographs, in the first edition (1989) of The Chlamydomonas
Sourcebook, pages 42-47.  A shorter version of the same text appears
in the second edition, volume 1, pages 255-256.


Elizabeth H. Harris

Chlamydomonas Center


Chlamy mailing list
Chlamy from net.bio.net

End of Chlamy Digest, Vol 46, Issue 1

More information about the Chlamy mailing list

Send comments to us at biosci-help [At] net.bio.net