[Chlamydomonas] Re: Chlamy Digest, Vol 46, Issue 1

Bernd, Karen via chlamy%40net.bio.net (by kabernd from davidson.edu)
Wed Sep 2 12:25:06 EST 2009


Frej-
It also depends on the purpose for replica plating. I have used 96 well plates for stock plates for years. I use a multichannel pipettor to fill the wells with whatever media you choose and a 96 pin applicator or an 8channel pipettor to transfer samples from an 'old' plate to a fresh one.

If you just need to transfer a small amount (dot blot/ lysing for enzymatic assay etc) this may also work, but Whatman paper is probably better. I know for certain that it cuts down stock maintenance time
    ******
     Karen K. Bernd, Ph.D.
Co-director Merck/AAAS Undergraduate Science Research Program
Associate Professor of Biology at Davidson College
      http://www.bio.davidson.edu/bernd

No trees were harmed in the typing of this email,
but thousands of electrons were terribly inconvenienced.
Please don't print this e-mail unless you really need to.



________________________________
From: <chlamy-request from oat.bio.indiana.edu>
Reply-To: <chlamy from oat.bio.indiana.edu>
Date: Wed, 2 Sep 2009 13:05:12 -0400
To: <chlamy from magpie.bio.indiana.edu>
Subject: Chlamy Digest, Vol 46, Issue 1

Send Chlamy mailing list submissions to
        chlamy from net.bio.net

To subscribe or unsubscribe via the World Wide Web, visit
        http://www.bio.net/biomail/listinfo/chlamy
or, via email, send a message with subject or body 'help' to
        chlamy-request from net.bio.net

You can reach the person managing the list at
        chlamy-owner from net.bio.net

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Chlamy digest..."


Today's Topics:

   1. replica plating (Frej Tulin)
   2. Re: replica plating (Elizabeth Harris)


----------------------------------------------------------------------

Message: 1
Date: Tue, 1 Sep 2009 18:02:12 -0400
From: Frej Tulin <frej.tulin from gmail.com>
Subject: [Chlamydomonas] replica plating
To: chlamy from magpie.bio.indiana.edu
Message-ID:
        <c8dd4ce40909011502u867b1a6hf6e6e9146b20077a from mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Dear all
Does anyone have a good method for replica plating Chlamydomonas plates? I
am currently using the standard budding yeast method with a velveteen. I
find that the colonies tend to 'smear out', which makes it difficult to
distinguish individual colonies, especially when the plate has a lot of
colonies.

Is there a filter paper or another material that gives better result?
Many thanks!
/frej


------------------------------

Message: 2
Date: Wed, 2 Sep 2009 07:30:27 -0400
From: Elizabeth Harris <chlamy from duke.edu>
Subject: Re: [Chlamydomonas] replica plating
To: chlamy from magpie.bio.indiana.edu
Message-ID: <p06200700c6c40722d5ed@[192.168.1.47]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

>Does anyone have a good method for replica plating Chlamydomonas plates?


Whatman #1 filter paper works much better for Chlamy than velveteen.
There's a detailed description of how to go about it, including
photographs, in the first edition (1989) of The Chlamydomonas
Sourcebook, pages 42-47.  A shorter version of the same text appears
in the second edition, volume 1, pages 255-256.


--

Elizabeth H. Harris

Chlamydomonas Center
http://www.chlamy.org/



------------------------------

_______________________________________________
Chlamy mailing list
Chlamy from net.bio.net
http://www.bio.net/biomail/listinfo/chlamy

End of Chlamy Digest, Vol 46, Issue 1
*************************************




More information about the Chlamy mailing list