[Chlamydomonas] motility question

Seppe Kuehn via chlamy%40net.bio.net (by skuehn from mail.rockefeller.edu)
Mon Jan 17 12:41:48 EST 2011


I am finding low levels of motility in logarithmically growing chlamy  
cells.  Motility levels are about 10-50% in mid-log cultures.  I am  
using the UTEX 2244 strain, cultured in Sager-Granick with the  
Hutner's trace metals.  Cells are grown at 25C & 7500 lux.  Mid-log  
densities in this medium are around 5e5/mL to 1e6/mL.  Is this  
common?  Non-motile cells in these cultures are generally in doublets  
or quadruplets.  They appear similar to the palmeloid state discussed  
in the chlamy handbook.  Any advice on getting higher motility - e.g.  
90% or more of cell swimming?

thank you,
Seppe
Rockefeller University



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