Mike-
First, I do o have a construct but, in looking for one do you want gfp or
rfp? For some reason I thought the autoflourescence in chlamy would make
rfp more easily detectible. I would love to hear what the list has!
Karen Bernd
On 10/4/11 1:03 PM, "chlamy-request from oat.bio.indiana.edu"
<chlamy-request from oat.bio.indiana.edu> wrote:
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>>>Today's Topics:
>> 1. gfp expression (Adams, Mike (Biology))
>>>----------------------------------------------------------------------
>>Message: 1
>Date: Tue, 4 Oct 2011 14:10:37 +0000
>From: "Adams, Mike (Biology)" <ADAMS from easternct.edu>
>Subject: [Chlamydomonas] gfp expression
>To: BioNet Chlamy <chlamy from magpie.bio.indiana.edu>
>Message-ID: <9A7E4BBF-6853-4D7F-9562-93F4AF8702A7 from easternct.edu>
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>>I have some undergraduates who would like to demonstrate transformation
>in Chlamy using gfp as the reporter. This would have to be a simple
>procedure as they will do it as part of a class. I have never transformed
>Chlamy, so I am wondering if there is a plasmid containing a constitutive
>gfp gene that can be introduced into Chlamy fairly easily.
>>Many thanks
>>Mike Adams
>adams from easternct.edu>>>>>>>------------------------------
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