[Chlamydomonas] CAPS marker for motility mutant

Darron Luesse via chlamy%40net.bio.net (by dluesse from siue.edu)
Mon Jan 2 11:20:10 EST 2012

Hi All,

I've been using the Mitchell and Graziano (2006) exercises in my Cell 
and Molecular Biology course for a couple of years, and the students 
seem to get a lot out of them.  I was hoping to expand a bit this 
semester and include some DNA work with Chlamy as well.  I am looking 
for a restriction digestion marker I can use to identify one of the 
common motility mutants (I typically use pf1, pf3, pf14, pf18, or ida4 - 
but would be open to others).  I'd like the students to be able to PCR 
amplify a segment of DNA, and then digest with an enzyme that would cut 
either mutant or wild-type.

Do any of you have a mutant/primer set/enzyme combo that you could 

Also, does anyone have a recommendation for a kit that they use to 
isolate Chlamy DNA?  I've looked into the cheap/easy protocols, but the 
lab space my class is in only has one fume hood for 20 students.  I have 
a feeling they'd get phenol all over the lab.

I'd appreciate any suggestions you could provide.  I'm a plant molecular 
biologist by trade and use Chlamydomonas as a teaching tool.  I'm still 
feeling my way around the wonderful world of Chlamy.

Darron Luesse

Darron R. Luesse Ph.D
Assistant Professor
Southern Illinois University Edwardsville
Department of Biological Sciences, Box 1651
Science Building 3326
Edwardsville, IL  62026
Phone:  (618) 650-2153
Fax:  (618) 650-3174
e-mail: dluesse from siue.edu

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