Heloisa,
I'm afraid I can't be much help if it is not contamination. It might be
that it is a cw- strain and your shaking is too rough - you're killing
them. You can try adding 50 mM sorbitol. I do have a question about your
EM: Do you happen to have the protocol you used to prep your samples for
EM? If so, do you mind forwarding it to me? I have recently gotten a
position at a teaching college and they have, of all things, an electron
microscope, so I would like to have the students working with it.
Thanks!
Take Care,
Sara G. Cline, Ph.D.
Athens State University
300 N. Beaty Street
Athens, AL 35611
256-233-6507
On Fri, Sep 27, 2013 at 12:03 PM, <chlamy-request from oat.bio.indiana.edu>wrote:
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>>> Today's Topics:
>> 1. Liquid culture growth (Heloisa Ciol)
>>> ----------------------------------------------------------------------
>> Message: 1
> Date: Fri, 27 Sep 2013 11:22:38 -0300
> From: Heloisa Ciol <helociol from gmail.com>
> Subject: [Chlamydomonas] Liquid culture growth
> To: chlamy from magpie.bio.indiana.edu> Message-ID:
> <CAFR92BG3eKTiHwr2Y2aiZbVeBi=
>zAi_OA0DEtUYEixQkVmHNdA from mail.gmail.com>
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>> Dear ones,
>> I'm reaching for you in hope you can help me.
>> I'm working with *Chlamydomonas reinhardtii* strain CC-4351 cw15 arg7-8 mt+
> [Matagne 325] and we've been facing some growth problems in liquid culture.
>> We've been harvesting the alga in TAP medium supplemented with Arginine,
> and it seems the culture does not reach the deep green colour expected
> after 4 or 5 days of growth (or even more - we've tried). Also the culture
> seems to be more viscous and "milky" then before.
>> We looked for contaminants in Birght Microscopy and some samples were even
> prepared to electronic microscopic, but we did not notice any contaminant
> within the Chlamydomonas cells.
>> We suppose there might be some problem with the media we are harvesting the
> alga, but don't know how to overcome this.
>> Do you have any suggestion of what it could be? Has someone faced the same
> problems brefore and could lend some help?
>> (I have pictures of the liquid culture and some microscopy photos, if it
> would help).
>> I appreciate your help.
>> Best regards,
>> --
> Heloísa Ciol
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