[Chlamydomonas] Re: Chlamy Digest, Vol 73, Issue 2

Sara Cline via chlamy%40net.bio.net (by sgcline from gmail.com)
Fri Sep 27 14:33:20 EST 2013


  I'm afraid I can't be much help if it is not contamination.  It might be
that it is a cw- strain and your shaking is too rough - you're killing
them.  You can try adding 50 mM sorbitol.   I do have a question about your
EM: Do you happen to have the protocol you used to prep your samples for
EM?  If so, do you mind forwarding it to me?  I have recently gotten a
position at a teaching college and they have, of all things, an electron
microscope, so I would like to have the students working with it.


Take Care,

Sara G. Cline, Ph.D.

Athens State University

300 N. Beaty Street

Athens, AL 35611


On Fri, Sep 27, 2013 at 12:03 PM, <chlamy-request from oat.bio.indiana.edu>wrote:

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> Today's Topics:
>    1. Liquid culture growth (Heloisa Ciol)
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> Message: 1
> Date: Fri, 27 Sep 2013 11:22:38 -0300
> From: Heloisa Ciol <helociol from gmail.com>
> Subject: [Chlamydomonas] Liquid culture growth
> To: chlamy from magpie.bio.indiana.edu
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> Dear ones,
> I'm reaching for you in hope you can help me.
> I'm working with *Chlamydomonas reinhardtii* strain CC-4351 cw15 arg7-8 mt+
> [Matagne 325] and we've been facing some growth problems in liquid culture.
> We've been harvesting the alga in TAP medium supplemented with Arginine,
> and it seems the culture does not reach the deep green colour expected
> after 4 or 5 days of growth (or even more - we've tried). Also the culture
> seems to be more viscous and "milky" then before.
> We looked for contaminants in Birght Microscopy and some samples were even
> prepared to electronic microscopic, but we did not notice any contaminant
> within the Chlamydomonas cells.
> We suppose there might be some problem with the media we are harvesting the
> alga, but don't know how to overcome this.
> Do you have any suggestion of what it could be? Has someone faced the same
> problems brefore and could lend some help?
> (I have pictures of the liquid culture and some microscopy photos, if it
> would help).
> I appreciate your help.
> Best regards,
> --
> Heloísa Ciol
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> End of Chlamy Digest, Vol 73, Issue 2
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