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Determination of on-rate and off-rate

George A. Heavner gheavner at voicenet.com
Tue Dec 19 23:18:13 EST 1995

I am working with a small peptide (MW < 500) that binds to a specific receptor.
I would like to determine both the on-rate and off-rate of the peptide with 
the receptor.  Both the purified receptor as well as cell lines that express 
the receptor are available.  I would appreciate suggestions or references on 
procedures that can be used to determine on- and off-rates.

Thanks in advance.

George A. Heavner
Senior Director, Peptide R&D
Centocor, Inc.

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