I am working with a small peptide (MW < 500) that binds to a specific receptor.
I would like to determine both the on-rate and off-rate of the peptide with
the receptor. Both the purified receptor as well as cell lines that express
the receptor are available. I would appreciate suggestions or references on
procedures that can be used to determine on- and off-rates.
Thanks in advance.
George A. Heavner
Senior Director, Peptide R&D