Tutorial -- "Image Restoration for 3-D Microscopy"

Ken Clark kwc at ibc.wustl.edu
Wed Jan 3 08:50:36 EST 1996


			    Tutorial Announcement

	           "Image Restoration for 3-D Microscopy"

	When:	Saturday, April 27, 1996;   08:00 to 17:00
		(Demonstrations and a social mixer:  evening prior)

	Where:	Washington University,
		Institute for Biomedical Computing
		St. Louis, Missouri  USA

	Audience: Biologists who are currently using or considering
		  use of image restoration or deconvolution methods
		  for 3-D fluorescence microscopy.

	Cost:	$25

	Maximum number of participants:	30

	Additional information: http://ibc.wustl.edu/bcl/cosm_tut.html

	Enquiries:	kwc at ibc.wustl.edu
	--------------------------------------------------------------
			Course	Outline

	Perspective and Goals
		Confocal Microscopy
			Fully Confocal
			Partial Confocal
		Computational 3-D Microscopy
			3-D Fluorescence
			Partially Confocal
		New Technologies

	Point-Spread Function (PSF) & Image formation
		PSF Definition
		PSF Measurement
		Theoretical PSF Computation
		Theoretical and Measured PSF Trade-offs
		Aberrations

	Data Collection and Noise Sources 
		CCD cameras, SIT & I-SIT cameras, PMT's
		Sampling (x,y, and especially z)
		Exposure Time
			Photon Noise
			Bleaching
			Phototoxicity
			Fluorescence Saturation
		Imaging the Relevant Region: Truncated Data, Missing Data

	Image Restoration
		Convolution
		Restoration Algorithms
			Nearest Neighbor (e.g. Vaytek)
			Jansson-Van Cittert (e.g. Applied Precision)
			Constrained Least Squares (e.g. Scanalytics)
			Maximum Likelihood
			Blind Deconvolution
				(e.g. Noran Instruments, AutoQuant Imaging)
		Noise Assumptions
		Computing and Memory Requirements
 
	Comparison of Algorithms for Image Restoration
 
	Discussion of Methods For Viewing 3-D Data Sets
 
	Panel Session with Users of 3-D Microscopy
	--------------------------------------------------------------






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