Hello everyone,
I am new to using NIH Image so this might
be a silly question. I want to use Image to
quantify the amount of DNA in some samples. I
fit the calibration data to a Rodbard-type curve
and then use the gelplot macro to mark the lanes
and obtain areas for the bands of interest. Is
there a way for NIH Image to convert these areas
to the units used for calibration (e.g., ng DNA)
or are the gelplot macros used only for
determining ratios? Is there a macro that would
let you calibrate using the areas (instead of the
8-bit gray-scale values) under the peaks of the
bands used for calibration? Is it accurate enough
to use the gray-scale value (instead of the area)
of the band in question along with the calibration
curve to determine the amount of DNA (or whatever
else one is trying to quantify)?
Any help would be appreciated. Thank you
all for your time.
Sincerely,
Alex
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