[Computational-biology] DNA Fragmentation: The Critical First Step
in Next Generation Sequencing
Niels Kruize / KBiosciences
(by niels.kruize from kbioscience.co.uk)
Fri Oct 30 10:49:53 EST 2009
Topic:Controlled DNA Fragmentation: The Critical First Step
in Next Generation Sequencing
When: November 11, 2009
Time: 11:00a.m. EST
Duration: Presentation: 30 Minutes
Discussion: 30 Minutes
Hosts: Introduction: Jim Laugharn, Founder and CEO
Presentation: Hamid Khoja, Ph.D., Senior Applications Scientist
Registration: To register for the online event:
1. Click here
2. Click Register
3. On the registration form, enter your information and then click Submit.
You will receive a confirmation email and an event reminder.
What You Will Learn:
The important role of Controlled DNA Fragmentation in the sample preparation process for Next Generation Sequencing.
What DNA Fragmentation methods are commercially available today for Fragment and Mate-Pair Libraries.
How the Covaris AFA process works.
Additional key applications and services.
DNA fragmentation is the most critical sample preparation step required by all the currently available next generation sequencing platforms. The sensitivity and broader application depth of these platforms necessitates the utilization of an efficient, easy to use, high throughput capable, and highly reproducible DNA fragmentation technology.
Although nebulization, enzymatic digestion, hydrodynamic shearing, and sonication have been used to shear DNA, they all have significant disadvantages, which cause them to be a weak point in the workflow for next generation sequencing technologies. Drawbacks include thermal and sequence specific biased shearing, thermal degradation, sample loss, automation difficulties, and user-based issues.
The Covaris AFA process stands out as the preferred method for easily and reproducibly fragmenting DNA ranging from 100bp to 5kb in length.
In this meeting we will provide details of the Covaris AFA technology, and the distinct advantages it provides when compared to other DNA shearing methodologies. We will also present data to showcase the control and reproducibility of our validated protocols when fragmenting DNA in the 100bp to 5kb size range. Also discussed will be the use of AFA technology in chromatin fragmentation for ChIP-chip, ChIP-seq, and RNA fragmentation applications.
At Covaris, we believe that high value samples deserve controlled preparation, prior to committing to high cost analysis. Covaris' line of high-performance focused ultra-sonicators deliver industry-leading sample processing capability directly to the benchtop. We provide workstation-based instruments capable of extremely rapid and complete DNA/RNA/Chromatin fragmentation, tissue disruption, and non-contact isothermal homogenization of cell culture, tissue, and organisms for DNA, RNA, protein, and metabolites extraction. The Covaris family of instruments provides single through 96 sample preparation with scalable acoustic energy, capable of processing a wide range of sample types and volumes.
With over 850 installations, the Covaris proprietary and patented Adaptive Focused Acoustics (AFA) technology enables numerous non-contact, isothermal sample preparation processes for use in a broad range of applications including Genomics, Proteomics, and Drug Discovery research.
For more information about Covaris and AFA, please visit us at Covarisinc.com or on KBioscience.
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