Morphine and ELISA

Falk Fish ila2027 at ZEUS.DATASRV.CO.IL
Tue Apr 18 17:11:29 EST 1995


On 13 Apr 1995, Frederic Bourgaud wrote:

> Hi all,
> 
> I am starting a new research program dealing with morphine in plants. I am
> looking for a rapid/reliable ELISA test that would be adapted to this. I
> already know that ELISA tech.(Lexington, Kentucky) commercialises a kit
> called "generic opiate ELISA test". It's a competition test and involves
> polyclonal antobodies. It is not very specific to morphine as it crosses
> with all the related alkaloids. It costs about 90 US $ per plate.
> 
> Does anybody know another ELISA test, maybe based on monoclonal antibodies
> ? It is important to me to have a test more specific to morphine. Also any
> experience/reference on morphine quantification in plants with ELISA would
> be appreciated.
> 
> Also posted to the Biotech and Plant-TC discussion lists. Sorry for
> multiple postings.
> 
> Thanks
> 
> Fred (bourgaud at ensaia.u-nancy.fr)

For tests for morphine and other drugs of abuse, you can try the following:
 
Hanson Hog Biomedical Co., Taipei, Taiwan, Tel +886-2-7922808, Fax 
..7914624 
 
Immunotech Corp, Boston, MA, Tel +1-617-787-1010, Fax ..787-0315
 
Princeton Biomeditech, Princeton, NJ, Tel +1-908-274-1000, Fax ..274-1010
 
BiosPacific, Emeryville, CA, Tel +1-415-652-6155, Fax ..652-4531
 
Eucardio Laboratory, San Diego, CA, Tel +1-619-689-8719, Fax ..689-8719
 
(The above addresses are from my own database, so some or all may be
outdated).
 
As to the specificity of the assay, I think that in your case, less
specificity is preferable for initial screening.  Once you find a plant
with traces of your material, try to determine the exact molecule.
 
Falk Fish, Tel-Aviv, Israel.
 
 




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