Freezing thymocytes: possible?

RAMPITSCH at BCRSSU.AGR.CA RAMPITSCH at BCRSSU.AGR.CA
Wed Apr 19 16:49:48 EST 1995


I am planning to use mouse thymocytes as feeder cells for hybridomas.
The thymus donors are now a month old, but the fusion... well I haven't
even started immunizing yet. I was wondering whether it is possible to
freeze feeder cells and thaw them a day or so before the fusion. I'd 
really rather use thymocytes than spleenocytes, so I have to decide
on this within the next week or so. Please let me know if you've tried
this, how it worked, protocols, etc.
Thanks,

Chris <Rampitsch at bcrssu.agr.ca>



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