lambda phage mabs

[Falk Fish]

On 21 Apr 1995, Robert Burns wrote:

> 
> Hi all,
>      have any of you had a go at generating antibodies using 
> lambda phage expression vectors. I know that one main advantage
> is the numbers of antibodies generated but what are the disadvantages?
> Thirdly does anyone know of an easy-to-use kit which can be used for
> this technology?
> 
> Thanks
> 
> Robert
> 
> 
> 
> 
>    
> Robert Burns
> 
The main problem of this method is the low affinity of the resulting 
antibodies.

I did not have direct experience with the method but we keep it in the 
back of our minds as a viable strategy to generate antibodies, while 
combining it with an approach to ensure that the affinity will be usably 
high.

As to a kit: I think Pharmacia offers a kit.  I am not sure and I will 
get back after looking at my papers.

You could also contact the guys at Cambridge: they have invented it all.

Falk Fish, Tel-Aviv, Israel