Storage of conjugates

Torsten Boerchers borcher at uni-muenster.de
Tue Aug 8 12:46:38 EST 1995


b3748 at cts.com (Bryan Kiehl) wrote:
>In article <199508010812.BAA11589 at net.bio.net>,
>   vigfrid.ness at veths.no (Vigfrid Ness) wrote:
>>Hi
>>Can anybody tell me the best way to store conjugated antibodies????
>>
>>Viffi Ness
>>DYNAL AS
>>
>Conjugates (alkaline phosphatase or peroxidase), assuming they were prepared 
>properly, can be stored at refrigeration temperatures for many months and are 
>stable frozen for years. In most cases, the reason for eventual inactivity is 
>microbial contamination.
>
>Bryan Kiehl
>GenBio
>15222-A Avenue of Science
>San Diego, CA 92128
>(619) 592-9300, ext 309
>(619) 592-9400 [FAX]

IMHO storage conditions cannot be generalized. They
may as well depend on the antibody, the stoichiometry of
conjugation and the method of coupling.

But remember, do not use sodium azide when storing peroxidase
conjugates!! A good alternative in this case is Thimerosal.

Torsten
-------------------------------------------------------------------------/
                           Torsten Boerchers                            /
    _/  _/_/_/ _/_/_/     Institute of Chemical- and Biochemical Sensor/
   _/  _/     _/   _/    Research (Molecular Biology Group)           /
  _/  _/     _/_/_/     Mendelstr 7, D-48149 Muenster, Germany       /
 _/  _/     _/    _/   Fax: +49-251-980 2890 Phone: +49-251-980 2876/
_/  _/_/_/ _/_/_/_/   E-Mail: borcher at uni-muenster.de              /
------------------------------------------------------------------/





More information about the Diagnost mailing list