conjugate stability

Martin Blankfard Mblank at kpl.com
Mon Aug 7 10:23:36 EST 1995


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>From: burns at sasa.gov.uk (Robert Burns)
>Subject: storage of conjugates
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>We routinely make monoclonal AP conjugates by thiolation of the
>antibody followed by reaction with activated AP. We find that thge
>storage conditions for each conjugate is dependent on the Mab used.
>
>Some conjugates store well at 4C, some don't. All of them store well
>at -20C. We have also been looking at a Pierce product called 
>superfreeze which allows liquid storage of conjugates at -20C which
>should increase longevity. We find that some conjugates store better
>with it and some without. BTW in some cases conjugates stored with
>superfreeze had decreased activity compared to aliquots of the same
>batch stored at 4C without it!
>
>I think with Mab conjugates it all depends on the parent antibody.
>
>
>Anybody else got any conjugate stories?
>
>
>Robert
>
>
>
>Robert Burns
>
>Monoclonal Antibody Unit
>Scottish Agricultural Science Agency
>East Craigs
>Edinburgh
>Scotland
>
>burns at sasa.gov.uk
>


Most conjugates can be made stable at 4C with 50% Glycerol in Picopure water. 
This works for both poly and monoclonal conjugates with a variety of enzymes 
as long as the protein concentration is at or above 1mg/ml.  Another 
reasonable stabilizer is 1% fish gelatin.  Fat oxidation and free radicals can 
be important when obtaining fish gelatin.

Martin Blankfard
Senior Scientist
Kirkegaard and Perry Laboratories
2 Cessna Ct. 
Gaithersburg Md , 20879
Phone: (US) 1-800-638-3167 x141
FAX : 301 948 9442
E-mail: Mblank at kpl.com
08/07/95
11:41:57

 



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