PCR KIT-> NO GELLS, Quantitative, Double Fidelity

Luc Ferrari ferrari at ctrmed.u-nancy.fr
Fri Dec 8 03:42:42 EST 1995


gwade at physiology.uwo.ca  <gwade at physiology.uwo.ca> wrote:

>>> I just found a great new product from Kalyx Biosciences.  AMPLISCRIPT 
>>> allows you to detect your PCR products using an ELISA format.  
>No labelled primers.
>Works on 96 welled plates.
>So now you have many copies of RNA which is complementary to the DNA
>you were probing for.
>
>Kalyx has some literature outlining this process.
>
>Interestingly enough they also have an inexpensive matrix on which you
>can do a nucleic acid hybridization in about 90 min (at room temp).
>
Hi,
The process seems interesting, but can it be used for the detection of small
PCR fragments, 100 to 300 pb, used to detect mRNA ?
And can it be used to quantified finally the mRNA ?

Luc

Luc Ferrari
Centre du Medicament - URA CNRS 597
Faculte de Pharmacie - Universite Henri Poincare                         
Nancy, France



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