gwade at physiology.uwo.ca <gwade at physiology.uwo.ca> wrote:
>>> I just found a great new product from Kalyx Biosciences. AMPLISCRIPT
>>> allows you to detect your PCR products using an ELISA format.
>No labelled primers.
>Works on 96 welled plates.
>So now you have many copies of RNA which is complementary to the DNA
>you were probing for.
>>Kalyx has some literature outlining this process.
>>Interestingly enough they also have an inexpensive matrix on which you
>can do a nucleic acid hybridization in about 90 min (at room temp).
The process seems interesting, but can it be used for the detection of small
PCR fragments, 100 to 300 pb, used to detect mRNA ?
And can it be used to quantified finally the mRNA ?
Centre du Medicament - URA CNRS 597
Faculte de Pharmacie - Universite Henri Poincare