Thermostable enzymes

Dr Kevin O'Donnell odonnell at sasa.gov.uk
Thu Dec 28 08:52:12 EST 1995


PCR and LCR are based on thermostable DNA polymerase and ligase 
respectively.  In the dog days between Christmas and New Year I find 
myself wondering what other sequence-specific amplification methods 
could be devised if suitable enzymes were available.  Can anyone tell me
 if there is, for example,  a thermostable RNA polymerase commercially
 available?  It seems to me that this would greatly simplify 
3SR/NASBA.

Input/ other ideas welcomed.

Kevin


Dr Kevin O'Donnell                           "Work as if you were in the early days
Diagnostics and Molecular Biology     of a better nation"
SASA                                                    - Alasdair Gray
Edinburgh



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