Thermostable enzymes

Richard Schifreen rschifre at access1.digex.net
Fri Dec 29 10:05:00 EST 1995


You are absolutely right as to the utility of thermostable enzymes for 
working with RNA; and that there currently aren't any available.  We (Life 
Technologies/ GIBCO BRL) are trying to engineer a thermostable reverse 
transcriptase.  So far, we're still trying.  If you'd like, I'd be glad 
to put you in contact with the project leader.

Have a great 1996.

Rich Schifreen          phone: 301-840-4163
Life Technologies       fax:   301-670-1493
   E-Mail: rschifre at access.digex.net



On 28 Dec 1995, Dr Kevin O'Donnell wrote:

> PCR and LCR are based on thermostable DNA polymerase and ligase 
> respectively.  In the dog days between Christmas and New Year I find 
> myself wondering what other sequence-specific amplification methods 
> could be devised if suitable enzymes were available.  Can anyone tell me
>  if there is, for example,  a thermostable RNA polymerase commercially
>  available?  It seems to me that this would greatly simplify 
> 3SR/NASBA.
> 
> Input/ other ideas welcomed.
> 
> Kevin
> 
> 
> Dr Kevin O'Donnell                           "Work as if you were in the early days
> Diagnostics and Molecular Biology     of a better nation"
> SASA                                                    - Alasdair Gray
> Edinburgh
> 
> 



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