HELP - Protein Electrophoresis on Cellulose Acetate Foil
KPAT1 at OP1.UP.AC.ZA
Wed Jun 7 03:18:34 EST 1995
We have used the Beckman Microzone Electrophoresis system with their
CA foil for decades (and have been VERY satisfied). We are informed
that Beckman no longer make the foil (57 x 145 mm) and have found
another source (Merck markets the BDH product # 44319). This seemed
to be the answer to our prayers (those who run electrophoresis will
know that once you have a system that works - you hang on to it).
However, we cannot get good clearing of the BDH membrane for
densitometric scanning using the Methanol/Acetic Acid/Heat method
that worked on the Beckman foils.
We have contacted Merck repeatedly but they appear to have no
interest in finding the answer. We have tried a variation of
Methanol/Acetic acid concentrations as well as different temperatures
- all to no avail.
Is there anyone out there who has experience with this product who
can advise us?
Thanks, in anticipation of a flood of solutions (excuse the pun).
Fred Reyers (Prof & Head: Section of Clinical Pathology)
Department of Medicine
Faculty of Veterinary Science
P.Bag X04, ONDERSTEPOORT, 0110,
Rep. of South Africa
Tel: 27-12-529 8288
FAX: 27-12-529 8308 or 27-12-344 2107 (Home)
"The great tradgedies of science
are the slaying of beautiful hypotheses by ugly facts"
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