HELP - Protein Electrophoresis on Cellulose Acetate Foil

[Fred Reyers]

Diagnost boffins

We have used the Beckman Microzone Electrophoresis system with their 
CA foil for decades (and have been VERY satisfied). We are informed 
that Beckman no longer make the foil (57 x 145 mm) and have found 
another source (Merck markets the BDH product # 44319). This seemed 
to be the answer to our prayers (those who run electrophoresis will 
know that once you have a system that works - you hang on to it). 
However, we cannot get good clearing of the BDH membrane for 
densitometric scanning using the Methanol/Acetic Acid/Heat method 
that worked on the Beckman foils.
We have contacted Merck repeatedly but they appear to have no 
interest in finding the answer. We have tried a variation of 
Methanol/Acetic acid concentrations as well as different temperatures 
- all to no avail.

Is there anyone out there who has experience with this product who 
can advise us?

Thanks, in anticipation of a flood of solutions (excuse the pun).

Fred

Fred Reyers (Prof & Head: Section of Clinical Pathology)
Department of Medicine
Faculty of Veterinary Science
P.Bag X04, ONDERSTEPOORT, 0110,
Rep. of South Africa
Tel: 27-12-529 8288
FAX: 27-12-529 8308 or 27-12-344 2107 (Home)

                       "The great tradgedies of science
            are the slaying of beautiful hypotheses by ugly facts"
                              (Huxley)