Mabs from PAGE bands

[Robert Burns]

Hi all,
       my first posting to the "now permanent" diagnost list

My question: Has anybody raised Mabs using protein bands excised
from PAGE gels as the immunogen? If so can you use the gel band
itself as an implant without having to elute the protein?

Second question ( I have asked this before but for the benefit of
newcomers) Is anybody interested in Fungal diagnostics by serological
methods?


Robert





Robert Burns

Monoclonal Antibody Unit
Scottish Agricultural Science Agency
East Craigs
Edinburgh
Scotland

burns at sasa.gov.uk