Use of PVP in ELISA

Prachya Kongtawelert mdbci001 at CMU.CHIANGMAI.AC.TH
Mon Mar 20 20:16:47 EST 1995


As I have recalled, there was a paper concerning about the use of 
polyvinylpyrrolidone as a blocker in ELISA in Analytical Biochemistry, 1994.

*************

Prachya Kongtawelert, Ph.D.
Res Lab for Joint Dis.,
Dept of Biochem., Fac of Med.,
Chiangmai University, Chiangmai,
Thailand 50200
Tel No. 66-53-894188
Fax No. 66-53-894188, 217144
email:mdbci001 at chiangmai.ac.th

On 20 Mar 1995, Isla Browning wrote:

> As suggested by Bioreba we use polyvinylpyrrolidone in extraction and =
> 
> conjugate buffers when carrying out ELISA for detection of virus in 
> plant sap. I would be grateful if someone could enlighten me as to why =
> 
>  it is used . Is its action as a blocker or as an antioxidant or does =
> it have 
> some other function?
> 
> 
> Isla Browning
> SASA
> Edinburgh
> 8/3/95
> =01=01=01=01
> 
> 
> 
> 



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