Use of PVP in ELISA

Collin, Roger COLLINR at agresearch.cri.nz
Tue Mar 21 17:10:29 EST 1995


With regard  to using PVP there is a paper in Analytical Biochemistry 
208:397-99, 1993 on "PVP as a blocking agent in immunochemical studies" byJW 
Haycock.
This paper recommends the use of PVP in conjunction with Tween 20 in Western 
Blots as it gives a high signal to background ratio.  I use this combination 
in an indirect competitive ELISA for sporidesmin (a mycotoxin) and have 
found that it works very well.

Regards Dr Roger Collin
AgResearch, Hamilton, New Zealand



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