low boiling point

Kevin O'Donnell odonnell at sasa.gov.uk
Tue Mar 21 11:27:16 EST 1995


<<Your message>>

 >We would like to initiate some PCR based methods near Quito,
 >Ecuador at an altitude of 3050 m above sea level.  Water boils
 >here at 89 C which will not allow us to bring the vials up above
 >this temperature.  Our PCR machine has not yet arrived but we
 >would like to have some ideas on how this problem might be solved
 >before it does.
 >Many thanks
 >Greg Forbes
<<End of your message>>

What an interesting problem! I somehow missed this when it was first posted.  
My immediate thought is: would this still be a problem if you had one of those 
thermal cyclers with a screw down heated lid?  Also, if water boils at a lower 
temperature, will the Tm of your DNA primers be lower as well?

Wish I knew more physics.    

Kevin O'Donnell		     
Diagnostics and Molecular Biology
Scottish Agricultural Science Agency    
Edinburgh                         		odonnell at sasa.gov.uk          
      

















Kevin O'Donnell		     
Diagnostics and Molecular Biology
Scottish Agricultural Science Agency    
Edinburgh                         		odonnell at sasa.gov.uk          
      
















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